VIPcheck™ is an easy-to-use assay that enables rapid and reliable detection of all types of azole resistance in Aspergillus fumigatus culture. Extensive validation shows excellent test characteristics: sensitivity of 97% and specificity of 98%, even when performed by technicians not trained in mycology.
VIPcheck™ can be used in every clinical microbiology laboratory and helps to determine which isolates need full in vitro susceptibility testing.
Aspergillus fumigatus causes a range of diseases in humans including allergic syndromes but also acute invasive aspergillosis in immunocompromised patients. For the management of diseases we rely on azole drugs, including itraconazole, voriconazole and posaconazole. These agents are effective if the isolate is susceptible to the azole class of drugs.
At this stage the VIPcheck™ can only be used for research purposes.
Our mission is to advance the diagnosis of fungal diseases by development of diagnostic tests, expert advice and on-the-job training in order to improve outcome of for patients with fungal discases.
We are a team of academic professionals who have specialized in the diagnosis and management of fungal diseases. We recognize that the microbiological diagnosis of (invasive) fungal diseases is usually very difficult and that patients receive therapy without knowing which fungus is causing the disease. With the emergence of resistance to antifungal drugs the need to identify the pathogen and determine the susceptibility to the drug has become more important. We have gained expertise in managing fungal diseases and aim to make this expertise available to share with our colleges.
The VIPcheck™ method consists of a 4-wells plate, which contains agar supplemented with 3 medical azoles (Voriconazole, Itraconazole and Posaconazole, VIP) and a growth control. The assay is rapid, requires no specific technical skills, and detects all azole resistance mechanisms, and therefore can be used in every microbiology laboratory. VIPcheck™ results can be reliably read after one day if growth of Aspergillus fumigatus is visible, and in all isolates after 2 days of incubation at 35-37°C.
Multiple Aspergillus fumigatus colonies from a patients culture can be tested using a single VIPcheck™-plate, and if a mixed azole-susceptible and azole-resistant colonies are present, the azole-resistant colonies will grow. We recommend to determine the in vitro susceptibility (MIC) for all colonies that grow on any of the agars that contain an azole.
In a recent study, three patients are described with proven invasive aspergillosis due to a voriconazole-susceptible and voriconazole-resistant Aspergillus fumigatus co-infection.Read more »